GABAergic Projections from the Medial Septum Selectively Inhibit
Interneurons in the Medial Entorhinal Cortex
Alfredo
Gonzalez-Sulser
,
Daniel
Parthier
,
Antonio
Candela
, and
5 more authors
Journal Of Neuroscience, Dec 2014
The medial septum (MS) is required for theta rhythmic oscillations and
grid cell firing in the medial entorhinal cortex (MEC). While GABAergic,
glutamatergic, and cholinergic neurons project from the MS to the MEC,
their synaptic targets are unknown. To investigate whether MSneurons
innervate specific layers and cell types in the MEC, we expressed
channelrhodopsin-2 in mouse MSneurons and used patch-clamp recording in
brain slices to determine the response to light activation of identified
cells in the MEC. Following activation of MS axons, we observed fast
monosynaptic GABAergic IPSPs in the majority (>60%) of fast-spiking
(FS) and low-threshold-spiking (LTS) interneurons in all layers of the
MEC, but in only 1.5% of nonstellate principal cells (NSPCs) and in no
stellate cells. We also observed fast glutamatergic responses to MS
activation in a minority (<5%) of NSPCs, FS, and LTS interneurons.
During stimulation of MS inputs at theta frequency (10 Hz), the
amplitude of GABAergic IPSPs was maintained, and spike output from LTS
and FS interneurons was entrained at low (25-60 Hz) and high (60-180 Hz)
gamma frequencies, respectively. By demonstrating cell type-specific
targeting of the GABAergic projection from the MS to the MEC, our
results support the idea that the MS controls theta frequency activity
in the MEC through coordination of inhibitory circuits.